ACUTE TOXICITY, ANALGESIC AND ANTIPYRETIC ACTIVITIES OF AN ‘INSOLUBLE’ EXTRACTIVE FROM THE STEMS OF CHENOPODIUM AMBROSIOIDES LINN

A. M. Lohdip, F. N. Ariahu, B. T. Fasanya

Abstract


Analyses were carried out on an insoluble extractive, a by-product of a previous study involving cold dilute acid hydrolysis of the stems of Chenopodium ambrosioides Linn using 0.1M HCl. (It is termed ‘Insoluble’ because it was found to be insoluble or just sparingly soluble in all solvents in which it was tested for solubility). The study involved the determination of acute toxicity (LD50) as well as testing for analgesic and antipyretic activities using Albino mice. Doses of 1000 mg/kg, 2000 mg/kg, 4000 mg/kg, 6000 mg/kg and 8000 mg/kg were administered intraperitoneally. The analgesic effect of the insoluble extract was tested using 1000 mg/kg, 2500 mg/kg and 3500 mg/kg using a hot plate maintained at 55 oC; with Pentazocine (100 mg/kg) and normal saline (0.1 mg/kg) as
positive and negative control, respectively. Antipyretic activity of the extract was also determined on the mice using 2000 mg/kg and 3000 mg/kg dosages, administered subcutaneously. Acetylsalicylic acid (Aspirin®) 100 mg/kg was used as standard. The LD50 was found to be 5000 mg/kg implying that it is safe. The analgesic activity showed very
significant inhibition of 164.4% and 199.85% inhibition for 2500 mg/kg and 3500 mg/kg, respectively. The extract showed antipyretic activity, when compared to the effect of the standard drug, aspirin®. These all imply that the extract is a promising starting material for analgesic and antipyretic drugs and it is safe.

Full Text:

PDF

Refbacks

  • There are currently no refbacks.