ANTIBIOTIC SUSCEPTIBILITY PROFILE OF ESCHERICHIA COLI SEROTYPE O157:H7 FROM RIVER KADUNA, NIGERIA

C N Okafor, S E Yakubu, J B Ameh, C A Okuofu

Abstract


In Nigeria, river water serves as raw material treated for drinking, recreation, irrigation and herd watering. This

river water is majorly contaminated with fecal samples from humans and animals introducing enteric microbes

including E. coli O157:H7. Although different epidemiological findings around the globe have linked E. coli

serotype O157:H7 to significant mortality and morbidity, the limitations associated with early diagnosis of this

pathogenic microbe is the clinical presentation and laboratory studies of this organism such as lower

gastrointestinal bleeding from neoplastic or diverticular disease, making it unpredictable and complicates the

diagnosis. This study evaluated the occurrence and antibiotic susceptibility profile of this E. coli pathotype from

river waters in Kaduna, Nigeria. Samples were collected using standard microbiological technique and antibiotic

susceptibility profile was carried out using agar disc diffusion method. The result showed that out of 204 water

samples collected for the period of one year, a total of 59 E. coli isolates were detected, out of which 25.4% (15)

of the isolates were of serotype O157:H7. However, the incidence of E. coli O157:H7 in the entire water samples

tested was 7.4% (15/204). The antibiotic susceptibility profile showed that the isolates were resistant to

Amoxicillin (73%), Sulphamethoxazole-Trimethoprim (66.7%) and Doxycycline (66.7%) while susceptible to

Amikacin (100%), Nitrofurantoin (100%) and Ceftriazone (100%). High percentage (73.3%) of the isolates had

MARI ≤ 2. The most common resistant pattern among the evaluated isolates was resistance to betalactam and

tetracycline groups of antibiotics. This study showed that though resistance was observed against some selected

antibiotics by E. coli O157:H7, Amikacin, Nitrofurantoin and Ceftriazone could still be used for the treatment of

this pathogenic microbe during disease outbreak.

Keywords: food/water-borne disease; antibiotics; E. coli O157:H7; River Kaduna


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